Triacylglycerol Levels in Fly Samples

The level of triacylglycerol (TAG) was measured as previously described [68 (link)]. Newly eclosed flies were pretreated with 25 μg/mL KRG for 10 days. Heat-treated homogenized samples from 20 flies were used for the measurement of TAG and free glycerol levels by using TAG reagent (T2449, Sigma-Aldrich) and free glycerol reagent (F6428, Sigma-Aldrich). Since body weight can be easily affected by water content, TAG level was normalized to the protein content.

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Lee S.H., Lee H.Y., Yu M., Yeom E., Lee J.H., Yoon A., Lee K.S, & Min K.J. (2019). Extension of Drosophila lifespan by Korean red ginseng through a mechanism dependent on dSir2 and insulin/IGF-1 signaling. Aging (Albany NY), 11(21), 9369-9387.

Publication 2019

TAG reagent free glycerol reagent F6428

Body weight Flies Glycerol Protein Triacylglycerol

Corresponding Organization :

Other organizations : Inha University, Korea Research Institute of Bioscience and Biotechnology

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Variable analysis

independent variables

Pretreatment with 25 μg/mL KRG for 10 days

dependent variables

Triacylglycerol (TAG) level
Free glycerol level

control variables

Protein content (used to normalize TAG level)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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