Immunohistochemical Quantification of Lymphocyte Subsets in IPMN

The FFPE tissue samples were cut into 4 mm thin sections. Slides were incubated for 10 min in 0.3% hydrogen peroxide. Subsequently, sections were incubated overnight at 4°C with the primary antibody. Then, slides were incubated with a secondary antibody [either EnVision + System-HRP Labeled Polymer anti-rabbit or anti-mouse (Dako, Agilent, Santa Clara, United States)]. The average density (cells/mm²) of each lymphocyte subset was calculated from five 1 mm² regions within each tumor to calculate its average density of positive cells (Reuben et al., 2017 (link)). IPMN was confirmed in all tissue samples by H&E staining, and the grade of dysplasia was evaluated by two experienced pathologists (ZJY and HX).

Free full text: Click here

Huang X., Feng Y., Ma D., Ding H., Dong G., Chen Y., Huang X., Zhang J., Xu X, & Chen C. (2022). The molecular, immune features, and risk score construction of intraductal papillary mucinous neoplasm patients. Frontiers in Molecular Biosciences, 9, 887887.

Publication 2022

EnVision + System-HRP Labeled Polymer anti-rabbit or anti-mouse

Antibody Cells Hydrogen peroxide Mouse Pathologists Polymer Rabbit Thin sections Tissue Tumor

Corresponding Organization : Nanjing Medical University

Other organizations : Second Affiliated Hospital of Nanjing Medical University

Top 5 similar protocols

Variable analysis

independent variables

Incubation of FFPE tissue sections in 0.3% hydrogen peroxide for 10 minutes
Incubation of sections with primary antibody overnight at 4°C
Incubation of slides with secondary antibody [either EnVision + System-HRP Labeled Polymer anti-rabbit or anti-mouse (Dako, Agilent, Santa Clara, United States)]

dependent variables

Average density (cells/mm^2) of each lymphocyte subset calculated from five 1 mm^2 regions within each tumor
Grade of dysplasia evaluated by two experienced pathologists (ZJY and HX)

control variables

FFPE tissue samples cut into 4 mm thin sections

positive controls

IPMN confirmed in all tissue samples by H&E staining

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!