Neuroprotective effects of PTE on OGD/R

Hippocampal neuronal HT22 and astrocytoma U251 cells were cultured in Dulbecco's modified eagle medium (DMEM) with 10% fetal bovine serum (FBS) (37°C, 5% CO₂), which was replaced by FBS-free DMEM 24 h before the experiment. Culture media were changed every 2 days. Cell culture reagents were obtained from Gibco (Grand Island, NY, USA). PTE dissolved with DMSO was diluted in DMEM in advance (DMSO ≤ 0.1%). Before OGD/R, U251 cells were treated with PTE (2.5 or 5 μM) or vehicle for 4 h.

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Liu H., Wu X., Luo J., Wang X., Guo H., Feng D., Zhao L., Bai H., Song M., Liu X., Guo W., Li X., Yue L., Wang B, & Qu Y. (2019). Pterostilbene Attenuates Astrocytic Inflammation and Neuronal Oxidative Injury After Ischemia-Reperfusion by Inhibiting NF-κB Phosphorylation. Frontiers in Immunology, 10, 2408.

Publication 2019

Cell culture reagents

Astrocytoma Cell culture Cells Dmso Eagle Fetal bovine serum Neuronal

Corresponding Organization : Air Force Medical University

Other organizations : 82th Hospital of Pla

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Protocol cited in 4 other protocols

Variable analysis

independent variables

Concentration of PTE (2.5 or 5 μM)

dependent variables

Cell response to OGD/R

control variables

Culture conditions (37°C, 5% CO2)
Culture media (DMEM with 10% FBS, replaced with FBS-free DMEM 24h before experiment)
Culture media change frequency (every 2 days)
DMSO concentration (≤ 0.1%)

controls

Positive control: Not specified
Negative control: Vehicle (DMSO) treatment

Annotations

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