Hydrogel-based Thioflavin T Assay
Corresponding Organization :
Other organizations : Chinese Academy of Medical Sciences & Peking Union Medical College, Sichuan University
Variable analysis
- Heating the 200 μL hydrogel to a transparent liquid
- Mixing the hydrogel with 2 μL Thioflavin T (ThT) solution (1 mmol)
- Cooling the sample to room temperature to form hydrogels
- Fluorescence of the sample observed at 365 nm
- Fluorescence detected by a Cary Eclipse fluorescence spectrophotometer with excitation/emission wavelengths at 450/485 nm
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned in the provided information.
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