Lipid Droplet Accumulation Assay

The LD accumulation of the test compounds was evaluated according to the studies published by co-author Professor Chia-Hung Yen [1 (link)]. The LD accumulation was detected by BODIPY^® 493/503 dye (Thermo Fisher Scientific). LD accumulation was achieved by treating cells with OA conjugated to BSA. All data were analyzed with GraphPad Prism 6.01 software (La Jolla, CA, USA). One-way analysis of variance (ANOVA) followed by Tukey’s comparison test was used to compare differences between multiple groups. A p-value < 0.05 was considered statistically significant.

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Cheng J.H., Wu H.C., Yen C.H., Hwang T.L., Ko H.H, & Chang H.S. (2023). Chemical Constituents with Anti-Lipid Droplet Accumulation and Anti-Inflammatory Activity from Elaeagnus glabra. Plants, 12(16), 2943.

Publication 2023

BODIPY® 493/503 dye GraphPad Prism 6.01 software

Bodipy 493 503 Cells Chia Prism

Corresponding Organization : Kaohsiung Medical University Chung-Ho Memorial Hospital

Other organizations : Taipei Medical University, Chang Gung University, Chang Gung University of Science and Technology, Chang Gung Memorial Hospital

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Variable analysis

independent variables

Treatment with OA conjugated to BSA

dependent variables

LD accumulation

control variables

BODIPY® 493/503 dye (Thermo Fisher Scientific) for detection of LD accumulation
Data analysis using GraphPad Prism 6.01 software

controls

Positive control: Cells treated with OA conjugated to BSA to induce LD accumulation
Negative control: Not explicitly mentioned

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