Comprehensive Western Blotting and Cell Cycle Analysis

Western blotting was performed as described previously ^{8 (link), 61 (link)}, with the exception that Alexa Fluor secondary antibodies were used. All blots were developed using the Odyssey fluorescence image scanner and the band intensities were quantified using LI–COR software. Cell cycle analysis was performed using PI (propidium iodide) staining as described previously ^{8 (link)}, using the CycleTEST PLUS DNA reagent kit (BD Biosciences, San Jose, CA, USA; # 340242). The following antibodies were used in the study: anti–P53 (SCBT; sc–6243), anti–P21 (BD; #556431), anti–APAF1 (R & D; #MAB828), anti–PIG3 (abcam; #ab64798), anti–PUMA (CST; #4976); anti–Ash2L (Bethyl Labs; #A300-108A), anti–H3K4me3 (abcam; #ab1012), anti–Wdr5 (abcam; #ab56919); anti–RbBP5 (Bethyl Labs; #A300-109A), anti–RNAP II (SCBT; sc–899); anti–Serine5–CTD (abcam; #ab5131–50); anti–TAFII (SCBT; sc–735); anti–TFIIB (SCBT; sc–225); anti–TFIIF (SCBT; sc–235); anti–Tubulin (Sigma; #T5168), Alexa Fluor 680 goat anti–mouse IgG (Molecular Probes; #A21057) and Alexa Fluor goat anti–rabbit IgG (Molecular Probes; #A21076).