Total mRNAs were isolated from the human cerebral organoids or cells using Trizol reagent, then cDNA was synthesized using AccuPower RT-PCR PreMix (Bioneer). The qRT-PCR was performed using SYBR Green Real-time PCR Master Mixes (Invitrogen) under the following reaction conditions (35 cycles): denaturation at 95°C for 1 min, annealing at 58°C for 30 s, and extension at 72°C for 30 s. Primer sequences were described in Table 2 . The expression levels were normalized relative to the expression of the housekeeping gene GAPDH[27 (link)] using the comparative Ct–method 2−ΔΔCt.
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