Microbial Community DNA Extraction from Yak Rumen

Total DNA from the microbial community in the ruminal fluid from 24 yaks was extracted according to the instructions of the FastDNA^® Spin Kit for Soil (MP Biomedicals, Solon, OH, United States). The determination of DNA concentration and purity, primer selection for PCR amplification, and PCR amplification procedures were performed according to our previous study (Yi et al., 2022 (link)). The PCR products of the same sample were mixed, subjected to 2% agarose gel to recover the PCR products, and extracted using an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA). A Quantum Fluorometer (Promega, Madison, WI, USA) was used to detect and quantify the recovered products. The sequencing library was constructed and sequenced using the Illumina Miseq technique (Illumina, San Diego, CA, USA) in a manner consistent with our previous study (Yi et al., 2022 (link)). All raw sequences have been uploaded to the NCBI database with the accession number: PRJNA978645.