Multidrug-Resistant P. aeruginosa Isolates Characterization

Of the 721 MDR P. aeruginosa sent to CFASS for extended antimicrobial susceptibility testing over a 20-year period (2001–2020), one-tenth of the patient-unique isolates were randomly selected for this study. These isolates, stored in the bacterial preservation system MICROBANKTM (Pro-Lab Diagnostics, Richmond Hill, ON, Canada) at −80 °C, were plated onto Mueller-Hinton (MH) agar (Oxoid Ltd., Basingstoke, UK) and were identified using a Vitek^® MS (BioMérieux, Inc., Durham, NC, USA). Isolates were tested by CFASS and were classified as multidrug-resistant if there was acquired non-susceptibility to at least one agent in the ≥3 antimicrobial group [22 (link)]. The media used for minimum inhibitory concentration (MIC) and synergy testing included Mueller-Hinton agar and cation-adjusted Mueller-Hinton II broth (CAMHB). P. aeruginosa ATCC 27853 was used as a quality control strain to validate the MIC values.

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Okoliegbe I.N., Hijazi K., Cooper K., Ironside C, & Gould I.M. (2021). Antimicrobial Synergy Testing: Comparing the Tobramycin and Ceftazidime Gradient Diffusion Methodology Used in Assessing Synergy in Cystic Fibrosis-Derived Multidrug-Resistant Pseudomonas aeruginosa. Antibiotics, 10(8), 967.

Publication 2021

MICROBANKTM Mueller-Hinton (MH) agar Vitek® MS

Agar Antimicrobial Bacterial Diagnostics Minimum inhibitory concentration P aeruginosa Patient Preservation Strain Susceptibility

Corresponding Organization : Aberdeen Royal Infirmary

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Variable analysis

independent variables

Selection of one-tenth of the patient-unique isolates of MDR P. aeruginosa from the 721 sent to CFASS for extended antimicrobial susceptibility testing over a 20-year period (2001–2020)

dependent variables

Classification of isolates as multidrug-resistant based on acquired non-susceptibility to at least one agent in the ≥3 antimicrobial group

control variables

Isolates were stored in the bacterial preservation system MICROBANK™ at −80 °C
Isolates were plated onto Mueller-Hinton (MH) agar and identified using a Vitek® MS
Media used for minimum inhibitory concentration (MIC) and synergy testing included Mueller-Hinton agar and cation-adjusted Mueller-Hinton II broth (CAMHB)
P. aeruginosa ATCC 27853 was used as a quality control strain to validate the MIC values

controls

P. aeruginosa ATCC 27853 was used as a positive control to validate the MIC values

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