The total RNA of cells and tissues were extracted from Trizol (Cat#9109; TaKaRa, Japan), and the PrimeScript RT Master Mix kit was used to reverse it into cDNA (Cat#RR047A; Takara, Japan). All of the mRNA levels were determined by employing the SYBR Green PCR Mix (Cat#RR420A; Takara, Japan) in conjunction with the CFX Connect (Bio-Rad, USA) as previous study [44 (link)]. The 2−ΔΔCT approach was utilized for data analysis, and each experiment was carried out with three separate sets of controls. The primer sequences used in this work were all synthesized by Sango Biotech (Shanghai, China), and they were all listed in the Table 1 .
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