HiChIP experiments were performed on shoots and roots of 14-d-old seedlings using the same procedure as in Concia et al. (2020) (link) with either anti-H3K9ac (MilliporeSigma 07–352) or anti-H3K27me3 (Sigma-Aldrich 07–449). The quality of the libraries was assessed with Agilent 2100 Bioanalyzer (Agilent), and the libraries were subjected to 2 × 75 bp paired-end high-throughput sequencing by NextSeq 500 (Illumina).
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HiChIP Profiling of Histone Modifications
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Corresponding Organization :
Other organizations : Institut des Sciences des Plantes de Paris Saclay, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre National de la Recherche Scientifique, Université Paris-Saclay, University of Warwick, King Abdullah University of Science and Technology, École Normale Supérieure - PSL, Inserm, Université Paris Sciences et Lettres, Institut de Biologie de l'École Normale Supérieure, Université Clermont Auvergne, Clermont Université, Instituto de Agrobiotecnología del Litoral, National University of the Littoral, Institut Universitaire de France, Université Paris Cité
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Variable analysis
independent variables
- Tissue type (shoots vs. roots)
- Chromatin interactions detected by HiChIP
- Seedling age (14-d-old)
- Experimental procedure (same as Concia et al. 2020)
- Positive control: Not specified
- Negative control: Not specified
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