Bioactive Compounds of Hibiscus sabdariffa

Delphinidin-3-sambubioside (D3S), quercetin (QRC), and hibiscus acid (HA) were chosen based on information from several articles that suggest those biocompounds are common among regional variants of Hibiscus sabdariffa [2 (link),12 (link)]. The methodology for the following section of the study was a modification of an article published by Martinez-Esquivias et al. [15 (link)]. The SwissTargetPrediction website was utilized as a tool to identify potential molecular targets of DS3, QRC, and HA in human cells. Following this, a comprehensive list of potential targets was generated for each compound. The ShinnyGo 0.77 website was subsequently employed to compute the fold enrichment (FE) of each target, with a false discovery rate (FDR) threshold established at 0.05. Among these targets, those exhibiting an FE value greater than 4 were utilized to ascertain the pathways through which interactions induced by these biological compounds occur. This was accomplished through the utilization of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Moreover, the Stringdb website was employed to retrieve the hub of genes derived from the FE data and to construct a Protein-to-Protein interaction network (PPI). This process is also shown in Figure 1. Finally, an extensive search was conducted using the Pubmed database to obtain relevant evidence.

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Zúñiga-Hernández S.R., García-Iglesias T., Macías-Carballo M., Pérez-Larios A., Gutiérrez-Mercado Y.K., Camargo-Hernández G, & Rodríguez-Razón C.M. (2024). Targets and Effects of Common Biocompounds of Hibiscus sabdariffa (Delphinidin-3-Sambubiosid, Quercetin, and Hibiscus Acid) in Different Pathways of Human Cells According to a Bioinformatic Assay. Nutrients, 16(4), 566.

Publication 2024

Corresponding Organization :

Other organizations : Universidad de Guadalajara

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Variable analysis

independent variables

Delphinidin-3-sambubioside (D3S)
Quercetin (QRC)
Hibiscus acid (HA)

dependent variables

Potential molecular targets of D3S, QRC, and HA in human cells
Fold enrichment (FE) of each target
Pathways through which interactions induced by these biological compounds occur

control variables

Control variables not explicitly mentioned

controls

Positive and negative controls not specified

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