Identifying Senescent Cells via β-Gal

The ß-Gal enzyme was detected in the fluorescein isothiocyanate (FITC) spectrum as described before [64 (link)]. The cells were incubated with bafilomycin A (Sigma-Aldrich, St. Louis, MO, USA) 100 nM for 1 h to induce lysosomal alkalization. The population with smaller size, complexity, and lower β-Gal activity was determined as NS. For RS and SIPS, the opposite characteristics were considered since β-Gal activity brightness was previously reported in SC [34 (link),36 (link)].

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Hernández-Mercado E., Prieto-Chávez J.L., Arriaga-Pizano L.A., Hernández-Gutierrez S., Mendlovic F., Königsberg M, & López-Díazguerrero N.E. (2021). Increased CD47 and MHC Class I Inhibitory Signals Expression in Senescent CD1 Primary Mouse Lung Fibroblasts. International Journal of Molecular Sciences, 22(19), 10215.

Publication 2021

bafilomycin A

Bafilomycin a Cells Enzyme Fluorescein Isothiocyanate Lysosomal

Corresponding Organization : Universidad Autónoma Metropolitana

Other organizations : Hospital de Especialidades, Universidad Panamericana, Universidad Anáhuac, Universidad Nacional Autónoma de México

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Variable analysis

independent variables

Incubation with bafilomycin A (100 nM for 1 h)

dependent variables

Lysosomal alkalization
Cell size
Cell complexity
β-Gal activity

control variables

Fluorescein isothiocyanate (FITC) spectrum for detecting β-Gal enzyme

Annotations

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