Microbial Genomic Analysis of Aromatic Hydrocarbon Degradation
As described in detail in the SI, ten single-end and two paired-end shotgun DNA libraries were constructed and sequenced with 454 and Illumina technology, respectively. Following quality control and assembly,20 (link)−22 assembled contigs and singletons longer than 200 bp were submitted to the Integrated Microbial Genomes and Metagenomics (IMG/M) system23 (link) for annotation. Functional profiling focused on the presence of genes for O2-independent and O2-dependent degradation of aromatic hydrocarbons, as well as of genes for methanogenesis and methane oxidation.
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An D., Caffrey S.M., Soh J., Agrawal A., Brown D., Budwill K., Dong X., Dunfield P.F., Foght J., Gieg L.M., Hallam S.J., Hanson N.W., He Z., Jack T.R., Klassen J., Konwar K.M., Kuatsjah E., Li C., Larter S., Leopatra V., Nesbø C.L., Oldenburg T., Pagé A.P., Ramos-Padron E., Rochman F.F., Saidi-Mehrabad A., Sensen C.W., Sipahimalani P., Song Y.C., Wilson S., Wolbring G., Wong M.L, & Voordouw G. (2013). Metagenomics of Hydrocarbon Resource Environments Indicates Aerobic Taxa and Genes to be Unexpectedly Common. Environmental Science & Technology, 47(18), 10708-10717.
Publication 2013
Aromatic hydrocarbons Dna librariesGenesMethane Methanogenesis Microbial genomes
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Other organizations :
University of Calgary, University of British Columbia, University of Alberta
Presence of genes for O2-independent and O2-dependent degradation of aromatic hydrocarbons
Presence of genes for methanogenesis and methane oxidation
control variables
Contigs and singletons longer than 200 bp
Quality control and assembly
Annotations
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