As described
in detail in the SI, ten single-end and
two paired-end shotgun DNA libraries were constructed and sequenced
with 454 and Illumina technology, respectively. Following quality
control and assembly,20 (link)−22 assembled contigs and singletons longer than 200
bp were submitted to the Integrated Microbial Genomes and Metagenomics
(IMG/M) system23 (link) for annotation. Functional
profiling focused on the presence of genes for O2-independent
and O2-dependent degradation of aromatic hydrocarbons,
as well as of genes for methanogenesis and methane oxidation.