Sanger Sequencing of FOXL2 and TERT

PCR amplification of FOXL2 and TERT promoter hotspot loci was performed using the AmpliTaq Gold 360 Master Mix kit (Life Technologies, ThermoFisher Scientific) using previously described primers [22 (link),23 (link)]. PCR fragments were cleaned using ExoSAP It (ThermoFisher Scientific) and Sanger sequenced as previously described [22 (link)].

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Da Cruz Paula A., da Silva E.M., Segura S.E., Pareja F., Bi R., Selenica P., Kim S.H., Ferrando L., Vahdatinia M., Soslow R.A., Vidal A., Gatius S., Przybycin C.G., Abu-Rustum N.R., Matias-Guiu X., Rubin B.P., Reis-Filho J.S., DeLair D.F, & Weigelt B. (2020). Genomic profiling of primary and recurrent adult granulosa cell tumors of the ovary. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(8), 1606-1617.

Publication 2020

AmpliTaq Gold 360 Master Mix kit ExoSAP It

Foxl2 Gold Primers Tert

Corresponding Organization : NYU Langone Health

Other organizations : Indiana University – Purdue University Indianapolis, Fudan University Shanghai Cancer Center, University of Genoa, Bellvitge University Hospital, Universitat de Barcelona, Centro de Investigación Biomédica en Red de Cáncer, Institut d'Investigació Biomédica de Bellvitge, Hospital Universitari Arnau de Vilanova, Universitat de Lleida, Cleveland Clinic

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Variable analysis

independent variables

PCR amplification of FOXL2 and TERT promoter hotspot loci

dependent variables

PCR fragments

control variables

AmpliTaq Gold 360 Master Mix kit (Life Technologies, ThermoFisher Scientific)
Previously described primers [22 (link), 23 (link)]
ExoSAP It (ThermoFisher Scientific)
Sanger sequencing as previously described [22 (link)]

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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