SARS-CoV-2 Spike Protein RBD Antibody Assay

SARS-CoV-2 RBD specific total antibody responses were determined using Sf9 insect cells SARS-CoV-2 spike protein RBD (GenScript, NJ, USA) as coating antigen and goat anti-mouse IgG HRP conjugate antibody (Jackson ImmunoResearch, Westgrove, PA, USA) as the detection antibody. The endpoint titers were determined by following the method, as described previously [16 (link)]. The cells secreting mouse IFN-γ by SARS-CoV-2-specific cells were evaluated using an ELISpot test for mouse IFN-γ (Mabtech, Stockholm, Sweden) by following the protocol as described previously [16 (link)], and the results are given in terms of spot-forming cells (SFCs)/10⁶ splenocytes.

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Panapitakkul C., Khorattanakulchai N., Rattanapisit K., Srisangsung T., Shanmugaraj B., Buranapraditkun S., Ketloy C., Prompetchara E, & Phoolcharoen W. (2022). Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice. Vaccines, 10(11), 1961.

Publication 2022

SARS-CoV-2 RBD SARS-CoV-2 spike protein RBD

Anti antibody Antibody Antibody responses Antigen Cells Elispot Goat Igg hrp Insect Mouse Mouse ifn γ Sars cov 2 Sars cov 2 spike protein Sf9 cells

Corresponding Organization : Chulalongkorn University

Other organizations : Thai Red Cross Society, King Chulalongkorn Memorial Hospital

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Variable analysis

independent variables

SARS-CoV-2 spike protein RBD (GenScript, NJ, USA)

dependent variables

SARS-CoV-2 RBD specific total antibody responses
Mouse IFN-γ secreting SARS-CoV-2-specific cells

control variables

Goat anti-mouse IgG HRP conjugate antibody (Jackson ImmunoResearch, Westgrove, PA, USA) as the detection antibody

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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