BMVEC Migration Assay with Modulators

Brain microvascular endothelial cells (BMVECs) were isolated as described previously (5 (link)). Experiments were performed using cells between passages 4 and 6. Cells were switched to serum-free medium 6 h before cell migration assay. The peroxynitrite decomposition catalyst 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron III chloride (FeTPPs; EMD Millipore) was used at a concentration of 5 µmol/L, whereas the nitration inhibitor epicatechin was used at a concentration of 200 μmol/L. Metformin was used at a 5 mmol/L concentration.

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Abdelsaid M., Prakash R., Li W., Coucha M., Hafez S., Johnson M.H., Fagan S.C, & Ergul A. (2014). Metformin Treatment in the Period After Stroke Prevents Nitrative Stress and Restores Angiogenic Signaling in the Brain in Diabetes. Diabetes, 64(5), 1804-1817.

Publication 2014

Brain Cell migration assay Cells Endothelial cells Epicatechin Iron chloride Metformin Nitration Peroxynitrite Serum

Corresponding Organization : University of Georgia

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Variable analysis

independent variables

FeTPPs (5 µmol/L)
Epicatechin (200 μmol/L)
Metformin (5 mmol/L)

dependent variables

Cell migration

control variables

Passage number of cells (between 4 and 6)
Serum deprivation period (6 h)

controls

Positive control: Not specified
Negative control: Not specified

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