Allonychiurus kimi colonies were first collected from Incheon, South Korea (32.267°N, 127.433°E) on 15 September 1996. Then, this collembolan population has been maintained at the Ecology & Toxicology Laboratory, Korea University, Seoul, South Korea (accession number KUETCOLC001) for approximately 25 years (Lee, Lee et al., 2021 (link)). The A. kimi colony was maintained in a plastic Petri dish (90 mm × 15 mm (diameter × height) (D × H)) filled with 0.5 cm deep substrate comprise of plaster of Paris, activated charcoal, and distilled water at a ratio of 4:1:4 by volume at 20 ± 1°C with continuous darkness (Wee, Lee, Kim, Son et al., 2021 (link)). Distilled water was added periodically, and the plastic Petri dishes were aerated weekly to maintain the fresh condition of the substrate. Brewer's yeast (Sigma‐Aldrich) was added weekly as food. To obtain cohorts of A. kimi, eggs laid by hundreds of adults on the same day were transferred to plastic Petri dish with a fresh moist substrate using a fine hair brush. The eggs were hatched after 10 days, and the juveniles were maintained under the same condition for 6 weeks until they develop into adults. Then, the adults were used for all subsequent experiments.
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