All animal experiments were conducted according to protocols approved by the Institutional Animal Care and Use Committee at Rutgers University (Piscataway, NJ). One hundred male C57BL/6J mice (age 5−6 wk) from either the departmental breeding colony or Jackson Laboratories were used in 2 long-term experiments. The first long-term treatment (Expt. 1, n = 38, 6 wk old) was designed to determine the effects of EGCG on BW and body fat in high-fat–fed mice. The 2nd long-term treatment (Expt. 2, n = 56, 5 wk old) was designed to confirm the findings on BW and fat from Expt. 1 and also to determine the effects of EGCG on fecal lipids, hyperglycemia, insulin resistance, markers of inflammation, and fatty liver caused by a high-fat diet. In both studies, mice were fed a LF, HF, or HFE diet for 16 wk. Based on allometric scaling, the presently used dose of 3.2 g/kg diet in mice corresponds to 10 200-mL cups of green tea (containing 2 g tea leaves per cup) per day for an average person requiring 8374 kJ (2000 kcal)/d. BW and food intake (on a per-cage basis) were measured weekly. For fecal lipid measurements from Expt. 2, cage bedding was changed during wk 10 and feces were collected from the cages the following day.