Histopathological Analysis of Formalin-Fixed CM Biopsies
Formalin-fixed, paraffin-embedded CM tissue biopsies were studied. Histopathological features were determined according to AJCC-UICC staging (40 (link)) (Table S1 in Supplementary Material). Proliferative index (PI) was determined by Ki-67+ staining (41 (link)) (%): Ki-67+ tumor cells/(Ki-67+ tumor cells + Ki-67− tumor cells) × 100. PI was determined in 1 mm2 tumor hot spot zone (clone MIB-1, Dako). HLA-I expression (%) was determined in tumor tissues with clone EMR8-5 (Abcam). The avidin–biotin–peroxidase (ABC) system (Vectastain, Vector Labs) was afterward used. Sections were examined by optical microscopy (Olympus BX40 microscope, DP2-BSW software), and digitalized pictures were analyzed with ImageJ software (NIH).
Mordoh J., Pampena M.B., Aris M., Blanco P.A., Lombardo M., von Euw E.M., Mac Keon S., Yépez Crow M., Bravo A.I., O’Connor J.M., Orlando A.G., Ramello F., Levy E.M, & Barrio M.M. (2017). Phase II Study of Adjuvant Immunotherapy with the CSF-470 Vaccine Plus Bacillus Calmette–Guerin Plus Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor vs Medium-Dose Interferon Alpha 2B in Stages IIB, IIC, and III Cutaneous Melanoma Patients: A Single Institution, Randomized Study. Frontiers in Immunology, 8, 625.
Corresponding Organization : Fundación para la Investigación, Docencia y Prevención del Cáncer
Other organizations :
Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto Alexander Fleming, Fundación Instituto Leloir, Instituto Oncológico de Córdoba
Formalin-fixed, paraffin-embedded CM tissue biopsies
controls
Positive control: Not specified
Negative control: Not specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
