Five single nucleotide polymorphisms (SNPs) in three genes associated with AMD or PCV were selected according to the literature, which were rs10468017 in LIPC[5 (link),6 (link),20 (link)]; rs3764261 in CETP[5 (link),6 (link)]; rs12678919 near LPL[5 (link),6 (link),20 (link)]. We also included LIPC rs1532085 and CETP rs173539, which regulated gene expression and showed impact on HDL levels[21 (link),22 (link)]. Blood samples were collected from all participants and stored at -80°C before DNA extraction. Genomic DNA was extracted from venous blood leukocytes using a genomic extraction kit (Beijing eBios Biotechnology Co., Ltd.), and genotyping was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), as previously described [23 (link)]. Briefly, approximately 30 ng of genomic DNA was used to genotype each sample. The DNA samples were amplified, and the PCR products were used for locus-specific single-base extension reactions. The resulting products were desalted and transferred to a 384 SpectroCHIP array. Allele detection was performed using MALDI-TOF-MS. The mass spectrograms were analyzed using MassARRAY Typer software version 4.0 (Sequenom, San Diego, CA, USA).
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