Glucose Tolerance Test Protocol

Glucose tolerance tests (GTTs) were performed as previously described^{43 (link)} with minor modifications. After a 6-h morning fast (ZT 0–ZT 6), basal plasma samples (t = 0) were collected at ZT 6, then mice were injected intraperitoneally with a 2 g/kg glucose bolus. Blood glucose was measured by tail bleed at set time points using a Bayer Contour Next EZ handheld glucometer (t = 15, 30, 45, 60, and 120 min). The study concluded after two hours at ZT 8. Insulin levels in plasma were measured using the STELLUX Chemiluminescent Rodent Insulin ELISA kit (ALPCO Diagnostics).

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Mushala B.A., Xie B., Sipula I.J., Stoner M.W., Thapa D., Manning J.R., Bugga P., Vandevender A.M., Jurczak M.J, & Scott I. (2023). G-protein coupled receptor 19 (GPR19) knockout mice display sex-dependent metabolic dysfunction. Scientific Reports, 13, 6134.

Publication 2023

Contour Next EZ STELLUX Chemiluminescent Rodent Insulin ELISA kit

Bleed time Blood glucose Diagnostics Elisa Glucose Glucose tolerance tests Insulin Mice Plasma Rodent Tail

Corresponding Organization :

Other organizations : University of Pittsburgh

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Variable analysis

independent variables

Intraperitoneal injection of a 2 g/kg glucose bolus

dependent variables

Blood glucose levels measured at set time points (15, 30, 45, 60, and 120 minutes)
Insulin levels in plasma

control variables

6-hour morning fast (ZT 0–ZT 6) prior to the glucose tolerance test
Time of day for the glucose tolerance test (ZT 6 to ZT 8)

controls

Basal plasma samples collected at t = 0 (ZT 6) before the glucose injection

Annotations

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