Synthesis and Characterization of N-Acyl Amino Acids

N-arachidonoyl glycine, N-arachidonoyl serine, and N-arachidonoyl-taurine were purchased from Cayman. N-arachidonoyl phenylalanine was purchased from Abcam. Arachidonic acid, anandamide, and WWL70 were purchased from Sigma-Aldrich. PF-3845 was purchased from Selleckchem. MAFP and EDTA were purchased from Fisher. Talabostat was purchased from R and D. Non-commercially available N-acyl amino acids were synthesized as previously described (Lin et al., 2018 (link); Long et al., 2016 (link)). Plasmids were obtained from the following sources: mouse PM20D1-flag (Addgene 132682), mouse FAAH-flag (Origene MR209084), mouse ACY1-flag (Origene MR206415), mouse CNDP1-flag (Origene MR219018), mouse CNDP2-flag (Origene MR207616), and mouse PM20D2-flag (MR222068).

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Kim J.T., Terrell S.M., Li V.L., Wei W., Fischer C.R, & Long J.Z. (2020). Cooperative enzymatic control of N-acyl amino acids by PM20D1 and FAAH. eLife, 9, e55211.

Publication 2020

N-arachidonoyl glycine Arachidonic acid anandamide WWL70 PF-3845 EDTA mouse PM20D1-flag

Amino acids Anandamide Arachidonic acid Cayman Edta Mouse N arachidonoyl glycine Pf 3845 Phenylalanine Plasmids Serine Talabostat Taurine Wwl70

Corresponding Organization :

Other organizations : Stanford University

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Variable analysis

independent variables

N-arachidonoyl glycine
N-arachidonoyl serine
N-arachidonoyl-taurine
N-arachidonoyl phenylalanine
Arachidonic acid
Anandamide
WWL70
PF-3845
Talabostat

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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