IHC staining of paraffin-embedded tissue array sections was conducted using the Envision System (Dako, Glostrup, Denmark), as described previously [19 (link)]. The primary antibodies used included anti-resistin mouse monoclonal antibody (clone C-10, diluted at 1 : 25; Santa Cruz Biotechnology, Santa Cruz, USA) and anti-fascin-1 mouse monoclonal antibody (clone 55k-2, diluted at 1 : 100; Santa Cruz Biotechnology).
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