Super-Resolution Microscopy Setup and Image Analysis

Images from two commercially available SIM microscopes were analysed, obtained on a Delta-Vision|OMX v4 by GE Healthcare (Issaquah, WA, USA) and on an Elyra S1 by Zeiss (Jena, Germany). Also, raw images were acquired on a home-built, SLM-based two-beam interference illumination SR-SIM microscope. This system consists of a 60 × , 1.2 numerical aperture water immersion objective (Olympus, Hamburg, Germany), a 642 nm, 85 mW fiber-coupled diode laser for excitation, a charge-coupled device camera (Coolsnap HQ, Photometrics, Tuscon, AZ, USA) and a liquid crystal display-based SLM for light modulation (LC-R 1920, Holoeye Photonics, Berlin, Germany). A sketch of the set-up can be found in Fig. 2e. The TIRF SR-SIM set-up is documented by Kner et al.5 (link).