The molecular weight (Mw) of pF1-20 was determined by HPGPC using an Agilent Technologies 1260 series (Agilent Technologies, Santa Clara, CA, USA) apparatus equipped with a Shodex OH-pak SB-804 HQ column (8 mm × 300 mm) and differential refractive index (RI) detector. Chromatographic conditions were performed as the previous method [18 (link),28 (link)]. The Mw was calculated by GPC software (Version 3.4) using a curve fitted by a serials of FG samples with known Mw (52.77, 39.9, 27.76, 14.92, 8.24, 5.30, 3.12 kDa).
The sulfate ester content of pF1-20 was measured by a conductimetric method [23 (link)]. The pFs (4 mg) was dissolved in 2.0 mL of distilled water. The solution was passed through a column (10 × 200 mm) of Dowex® 50WX8 cation exchange resin (H+ form), and the acid form of these samples was eluted with water and then the solution was titrated with 2 mM NaOH at room temperature, monitored by a conductivity meter (DDSJ-308A). The sulfate ester content was calculated from the conductivity titration curves.
The FI-IR spectra (KBr pellets) of SvFG and pF1–12 (~1 mg) were recorded on a Bruker Tensor 27 infrared spectrometer (Ettlingen, Germany) in the range of 400–4000 cm−1.
The sulfate ester content of pF1-20 was measured by a conductimetric method [23 (link)]. The pFs (4 mg) was dissolved in 2.0 mL of distilled water. The solution was passed through a column (10 × 200 mm) of Dowex® 50WX8 cation exchange resin (H+ form), and the acid form of these samples was eluted with water and then the solution was titrated with 2 mM NaOH at room temperature, monitored by a conductivity meter (DDSJ-308A). The sulfate ester content was calculated from the conductivity titration curves.
The FI-IR spectra (KBr pellets) of SvFG and pF1–12 (~1 mg) were recorded on a Bruker Tensor 27 infrared spectrometer (Ettlingen, Germany) in the range of 400–4000 cm−1.
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