Quantifying Kidney Injury Biomarker KIM-1

Histology was performed on sections from formalin-fixed kidneys as described in detail elsewhere [18 (link),22 (link)]. Stained sections were scanned with a 20× objective using a Scanscope AT slide scanner (Aperio, Leica Biosystems, Buffalo Grove, IL, USA) and quantitative image analysis was performed using Visiomorph software (Visiopharm, Hørsholm, Denmark). KIM-1 (AF1817, R&D systems, Minneapolis, MN, USA) was quantified in whole-kidney sections, expressed as proportional (%) surface area of immunopositive staining.

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Dalbøge L.S., Christensen M., Madsen M.R., Secher T., Endlich N., Drenic’ V., Manresa-Arraut A., Hansen H.H., Rune I., Fink L.N, & Østergaard M.V. (2022). Nephroprotective Effects of Semaglutide as Mono- and Combination Treatment with Lisinopril in a Mouse Model of Hypertension-Accelerated Diabetic Kidney Disease. Biomedicines, 10(7), 1661.

Publication 2022

Scanscope AT Visiomorph software KIM-1 (AF1817,

Buffalo Formalin Kidney Kim 1

Corresponding Organization : Gubra (Denmark)

Other organizations : Vifor Pharma (Switzerland)

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Variable analysis

independent variables

Histology performed on sections from formalin-fixed kidneys

dependent variables

Quantification of KIM-1 (AF1817, R&D systems, Minneapolis, MN, USA) expressed as proportional (%) surface area of immunopositive staining

control variables

Histology performed as described in detail elsewhere [18 (link), 22 (link)]
Stained sections scanned with a 20× objective using a Scanscope AT slide scanner (Aperio, Leica Biosystems, Buffalo Grove, IL, USA)
Quantitative image analysis performed using Visiomorph software (Visiopharm, Hørsholm, Denmark)

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