While under isoflurane anesthesia, blood was collected from each mouse into K3 EDTA micro tubes (Sarstedt, Nümbrecht, Germany). Each blood sample was centrifuged at 3000× g for 10 min at 4 °C to obtain the plasma. The levels of plasma cytokines were measured using a mouse cytokine/chemokine discovery assay (Eve Technologies, Calgary, AB, Canada) [15 (link)].
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