Dextran extravasation was performed as described previously35 (link),36 (link) with modification. Mice were injected intraperitoneally with 100 μL of fluorescently conjugated 10-kDa (Alexa Fluor-555; Invitrogen) and 70-kDa (Texas Red; Invitrogen) dextran tracers at a concentration of 3.75 mg/mL and returned to their home cages for 30 minutes to allow the dextrans to circulate. Animals were then transcardially perfused with TBS. Cortices were dissected, weighed, and incubated in formamide for 48 hours at 56 °C. Dye fluorescence was then measured using a SpectraMax i3× Multi-Mode Plate Reader (Molecular Devices) at the appropriate emission and excitation wavelength and normalized to tissue weight.
Protocol detail
Dextran Extravasation Quantification Protocol
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : University of Illinois at Chicago
Top 5 similar protocols
Variable analysis
independent variables
- Injection of 10-kDa (Alexa Fluor-555) and 70-kDa (Texas Red) dextran tracers at a concentration of 3.75 mg/mL
- Dye fluorescence measured using a SpectraMax i3× Multi-Mode Plate Reader (Molecular Devices) at the appropriate emission and excitation wavelength and normalized to tissue weight
- Mice were injected intraperitoneally and returned to their home cages for 30 minutes to allow the dextrans to circulate
- Animals were then transcardially perfused with TBS
- Cortices were dissected and weighed
- Cortices were incubated in formamide for 48 hours at 56 °C
- No positive or negative controls were explicitly mentioned in the protocol.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!