The rats were divided into sham, vehicle, MCAO, SC79, and SC79+LY294002 groups. 60 min prior to cerebral ischemia (60 min) followed by reperfusion, SC79 (S7863) (Selleck, Shanghai, China) was applied via intraperitoneal injection at a concentration of 0.04 mg/g of body weight. The mice were subjected to focal cerebral ischemia (60 min) followed by reperfusion (24 hours).
To determine the role of PI3K/Akt signaling in SC79-induced protection against cerebral I/R injury, the PI3K inhibitor LY294002 (Sigma, Shanghai, China) (1 mg/25 g body weight) was given to mice 15 min before SC79 administration according to the previous report [15 (link)]. Rats in the model and sham groups were injected with an equal volume of saline.
To determine the role of PI3K/Akt signaling in SC79-induced protection against cerebral I/R injury, the PI3K inhibitor LY294002 (Sigma, Shanghai, China) (1 mg/25 g body weight) was given to mice 15 min before SC79 administration according to the previous report [15 (link)]. Rats in the model and sham groups were injected with an equal volume of saline.
