Immunohistochemical Analysis of HMGCS2 in Mouse Lung

IHC analysis was performed as we previously described [17 (link)]. Briefly, mouse lung tissues were embedded in paraffin, sectioned, and rehydrated; these antigens were recovered by heating in relative buffer at 95 °C for 10 min following the antibody instructions (anti-HMGCS2 (1:200; Abcam ab137043)). Endogenous peroxidase was neutralized using the endogenous peroxidase-blocking solution. Afterwards, the lung sections were incubated with a primary antibody at 4 °C overnight. After biotin-labelled secondary antibodies were incubated at 37 °C for 30 min, the lung sections were developed with DAB working solution and then stained with hematoxylin and mounted with a mounting medium.