Transesterification of Organic Samples

Samples R1a, R1b and R2a were subjected to a transesterification procedure in accordance with a procedure previously reported in the literature [13 (link)]. In brief, about 100.0 mg of the samples was dissolved in 0.2 mL of MeOH. Then, MeONa was added (24.1 mg, 0.9 mmol) and the mixture was stirred for 3 h at room temperature. After this time, the crude compound was diluted with AcOEt (25 mL) and washed with water (3 × 10 mL). The combined organic layers were dried over Na₂SO₄, filtered, concentrated under a vacuum and then were subjected to GC/MS analyses. GC/MS analyses were performed with QP-2010-Plus Gas Chromatograph Mass Spectrometer (Shimadzu Italia S. r. l., Milan, Italy) using a “silica fusa” Rix^®-5ms column (Restel^®) (30 m, 0.25 mm ID, 0.25 µm) (Restel S.r.l., Cernusco Sul Naviglio, Milan, Italy), with a flow of 1.0 mL/min. The temperature varied from 100 °C to 250 °C in 10′.