Polydopamine Nanocarrier Synthesis and Characterization

Polydopamine nanocarriers are formed by the auto-polymerization of dopamine hydrochloride under appropriate conditions. Add 10 mL distilled water and 10 mL anhydrous ethanol into the same beaker and place them in a magnetic stirrer for stirring. During the stirring process, absorb 0.5 mL ammonia water and add it into the beaker, and continue to stir at constant temperature. Weight 100 mg dopamine hydrochloride powder, absorb 1 mL distilled water to dissolve it fully, add it to the beaker as soon as possible, keep the system stirred at a constant speed for 12 h, then centrifuge the beaker solution separately (12 000 rpm, 2 min), carefully remove the supernatant with a pipette, and repeat the operation for 5 to 7 times. After centrifugation, the supernatant was water transparent, indicating that the washing and purification of the polydopamine nanomaterial had been completed. After centrifugation, the precipitated polydopamine nanoparticles were used for subsequent curcumin load characterization and detection. The size distribution and polydispersity index of polydopamine nanoparticles were determined by dynamic light scattering (DLS) measurements (Nano ZS90, Malvern Instruments, UK).