Cultivation and Compound Preparation for Candida

Candida albicans strain SC5314 (Fonzi and Irwin, 1993 (link)) and C. glabrata strain BG2 (Kaur et al., 2007 (link)) were grown at 30°C on YPD [1% yeast extract, 2% bacteriological peptone (LabM, UK) and 2% glucose (Sigma-Aldrich, USA)] agar plates. Dimethyl sulfoxide (DMSO) (VWR International, Belgium) was used as a solvent to prepare stock solutions of miconazole (MCZ; Sigma-Aldrich), fluorescently labeled imidazole derivative (Benhamou et al., 2017 (link)), tetraethylammonium bromide (TCI Europe, Belgium), itraconazole (TCI Europe) and domiphen bromide (DB; Chemische Fabrik Berg GmbH). RPMI 1640 medium (pH 7.0) with L-glutamine and without sodium bicarbonate was purchased from Sigma-Aldrich and buffered with MOPS (Sigma-Aldrich). An ascorbic acid (Sigma-Aldrich) stock was made in Milli-Q water.