Immunohistochemical Analysis of MYH11

Protein distribution was assessed by immunohistochemistry as described (Lu et al., 2021a (link)). Uterine cryosections were 8 μm, and antibodies were mouse monoclonal antibody to MYH11 (ab683 clone 1G12, 1:200; Abcam) and Alexa Fluor 488-conjugated goat anti-mouse IgG (Cell Signaling Technology, dilution 1:500). Immunoreactivity was evaluated using a Leica TCS SP8 confocal laser scanning microscope system (Leica Microsystems Inc., Buffalo Grove, IL, United States).

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Qu M., Lu P., Bellve K., Lifshitz L.M, & ZhuGe R. (2021). Mode Switch of Ca2 + Oscillation-Mediated Uterine Peristalsis and Associated Embryo Implantation Impairments in Mouse Adenomyosis. Frontiers in Physiology, 12, 744745.

Publication 2021

ab683 clone 1G12 Alexa Fluor 488-conjugated goat anti-mouse IgG Leica TCS SP8 confocal laser scanning microscope system

Alexa fluor 488 Anti igg Antibodies Buffalo Clone Confocal laser scanning microscope Cryosections Dilution Goat Immunohistochemistry Monoclonal antibody Mouse Protein Uterine

Corresponding Organization : University of Massachusetts Chan Medical School

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Variable analysis

independent variables

Protein distribution assessed by immunohistochemistry

dependent variables

Immunoreactivity evaluated using a Leica TCS SP8 confocal laser scanning microscope system

control variables

Uterine cryosections were 8 μm
Mouse monoclonal antibody to MYH11 (ab683 clone 1G12, 1:200; Abcam)
Alexa Fluor 488-conjugated goat anti-mouse IgG (Cell Signaling Technology, dilution 1:500)

controls

Positive control: Not specified
Negative control: Not specified

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