Dietary Intervention in C57BL/6J Mice

Two studies were conducted under the Institutional Animal Care and Use Committee approved Animal Care and Use Protocol procedures at Mayo Clinic (Rochester, MN) and Bristol Myers Squibb (BMS, Lawrenceville, NJ). The investigative procedures regarding diet and treatment groups were conducted in the same manner at both sites, as follows: 6-week-old, male C57BL/6J mice were purchased from Jackson Laboratories (Bar Harbor, ME) and acclimated to either the BMS (single-housed, N = 36) or Mayo Clinic animal facilities (group-housed, 3–4 per cage, N = 42) for 1 week on standard rodent chow (BMS: Teklad, 2018, Madison, WI; Mayo Clinic: Lab Diet 5053 PicoLab^® Rodent Diet-20; St. Louis, MO). At 7 weeks of age, mice of normal body weight (~22–24 g) were randomized to receive either a matched control diet (CD) containing standard levels of choline, methionine, and fat (Research Diets, A13012807, New Brunswick, NJ) or a choline-deficient, amino acid-defined, (methionine, 0.1%), high-fat (60% of calories from fat) diet (CDAHFD; Research Diets, A06071302, New Brunswick, NJ).^{10 –12 ,33 (link)}