The morphology of the PLGA and PLA nanofibrous membranes in their pristine state (ie, uncoated membranes) was studied by scanning electron microscopy (SEM). The measurements were carried out in accordance with a previously published protocol:9 (link) the membranes were sputter-coated with gold and evaluated by SEM (Quanta 450; Thermo Fisher Scientific, Waltham, MA, USA) in high vacuum mode. The images were taken using an Everhart–Thornley detector in secondary electron mode at high voltage (20 kV) and magnification 2,000× and 10,000×. The thickness of the membranes was determined from SEM images of a vertical section of the membrane. The diameter of the fibers was measured on the SEM images using Atlas software (Tescan, Brno, Czech Republic).
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Characterization of PLGA and PLA Nanofibers
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Corresponding Organization : Czech Academy of Sciences, Institute of Physiology
Other organizations : Charles University, Hstar Technologies (United States), Czech Academy of Sciences, Institute of Macromolecular Chemistry, Czech Academy of Sciences, Institute of Rock Structure and Mechanics
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Variable analysis
independent variables
- PLGA and PLA nanofibrous membranes
- Morphology of the PLGA and PLA nanofibrous membranes
- Thickness of the membranes
- Diameter of the fibers
- Sputter-coating the membranes with gold
- Scanning electron microscopy (SEM) in high vacuum mode
- Everhart–Thornley detector in secondary electron mode
- High voltage (20 kV)
- Magnification 2,000× and 10,000×
- Atlas software (Tescan, Brno, Czech Republic) for fiber diameter measurement
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