Twenty-four rats were randomly divided into 4 groups: control, Letrozole, HFD, and Letrozol+HFD.
Control group (n=6): The rats were fed with a standard laboratory diet (3.85 kcal/g, energy supply ratio: protein 20%, carbohydrate 70%, fat 10%) and received vehicle only [0.5% of carboxymethyl cellulose (CMC) 2 ml/kg body weight] once daily p.o.
Letrozole group (n=6): The rats were administered Letrozole (Jiangsu Hengrui Medicine Co., China) at a concentration of 1 mg/kg p.o. dissolved in 0.5% CMC solutions once daily and fed with the same standard laboratory diets as the control group [22 (link)].
HFD group (n=6): The rats were fed with a high-fat diet (5.24 kcal/g, energy supply ratio: protein 20%, carbohydrate 20%, fat 60%) and received the same vehicles as the control group.
Letrozol+HFD group (n=6): The rats were administered similar Letrozole as in the Letrozole group and fed with the same high-fat diet as the HFD group.
All the treatment periods were 12 weeks. At the end of the experiment, all rats were sacrificed by decapitation for evaluation.
Control group (n=6): The rats were fed with a standard laboratory diet (3.85 kcal/g, energy supply ratio: protein 20%, carbohydrate 70%, fat 10%) and received vehicle only [0.5% of carboxymethyl cellulose (CMC) 2 ml/kg body weight] once daily p.o.
Letrozole group (n=6): The rats were administered Letrozole (Jiangsu Hengrui Medicine Co., China) at a concentration of 1 mg/kg p.o. dissolved in 0.5% CMC solutions once daily and fed with the same standard laboratory diets as the control group [22 (link)].
HFD group (n=6): The rats were fed with a high-fat diet (5.24 kcal/g, energy supply ratio: protein 20%, carbohydrate 20%, fat 60%) and received the same vehicles as the control group.
Letrozol+HFD group (n=6): The rats were administered similar Letrozole as in the Letrozole group and fed with the same high-fat diet as the HFD group.
All the treatment periods were 12 weeks. At the end of the experiment, all rats were sacrificed by decapitation for evaluation.
