Lectin-MASP-2 Complex Activity Assay

Activity of lectin(s)-MASP-2 complexes was determined as previously described (43 (link)) with modification (44 (link)). LPS from various bacteria were used for coating of microtiter plates (Maxisorp U96, Nunc). The products of C4 activation were detected with rabbit anti-hC4c and HRP-conjugated anti-rabbit Ig (Dako). To test MBL-MASP-1 complex activity, VPR-AMC (Val-Pro-Arg-aminomethylcoumarin) peptide (Bachem, Switzerland), as the substrate for MASP-1 was used as previously described (45 (link)) and the fluorescence was read using a Varioskan Flash reader (Thermo Scientific, USA).

Free full text: Click here

Man-Kupisinska A., Swierzko A.S., Maciejewska A., Hoc M., Rozalski A., Siwinska M., Lugowski C., Cedzynski M, & Lukasiewicz J. (2018). Interaction of Mannose-Binding Lectin With Lipopolysaccharide Outer Core Region and Its Biological Consequences. Frontiers in Immunology, 9, 1498.

Publication 2018

HRP-conjugated anti-rabbit Ig VPR-AMC Varioskan Flash reader

Bacteria Fluorescence Lectin Masp Masp 2 Peptide Pro arg Rabbit

Corresponding Organization : Polish Academy of Sciences

Other organizations : University of Łódź

Top 5 similar protocols

Variable analysis

independent variables

LPS from various bacteria used for coating of microtiter plates

dependent variables

Activity of lectin(s)-MASP-2 complexes
Products of C4 activation
MBL-MASP-1 complex activity

control variables

Maxisorp U96 microtiter plates (Nunc) used for coating
Rabbit anti-hC4c and HRP-conjugated anti-rabbit Ig (Dako) used for detecting products of C4 activation
VPR-AMC (Val-Pro-Arg-aminomethylcoumarin) peptide (Bachem, Switzerland) used as the substrate for MASP-1

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!