All experiments were done in accordance with the rules of the Ethics Committee of Medical Faculty (EC/KNRC/90-2; Kerman University, Iran). A total of 35 male rats (NMRI, 230 to 275 g) were kept in the animal cage with free access to food and water. Animals were randomly divided into 5 groups before Traumatic Brain Injury (TBI) induction: Sham (intact) (n=7), TBI group (n=7), vehicle (n=7) that exposed to TBI and received an intraperitoneal injection of melatonin vehicle (ethanol + normal saline; 0.33 mL/rat) (Chern, Liao, Wang, & Shen, 2012 (link); Dehghan, Hadad, Asadikram, Najafipour, & Shahrokhi 2013 (link)), and two TBI+melatonin groups that were exposed to TBI and then received 5 mg/kg and 20 mg/kg melatonin (Sigma, St. Louis, MO), respectively at 1 hour, and then 1, 2 and 3 days post-TBI (Babaee et al., 2015 (link); Gutierrez-Cuesta et al., 2007 (link)).
Protocol detail
Melatonin Effects on Traumatic Brain Injury
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Animals
Ethanol
Ethics committee
Food
Intraperitoneal injection
Male
Medical faculty
Melatonin
Nmri
Normal saline
Post traumatic brain injury
Traumatic brain injury
Corresponding Organization :
Other organizations : Kerman University of Medical Sciences, Rafsanjan University of Medical Sciences
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Variable analysis
independent variables
- Traumatic Brain Injury (TBI) induction
- Melatonin administration (5 mg/kg and 20 mg/kg)
- Not explicitly mentioned
- Animal species (NMRI male rats)
- Animal weight (230 to 275 g)
- Animal housing (animal cage with free access to food and water)
- Positive control: Sham (intact) group
- Negative control: Vehicle group (exposed to TBI and received melatonin vehicle)
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