ChIP assay was performed using the Zymo-Spin ChIP kit according to the manufacturer’s protocol (Zymo Research). Briefly, FLS were serum starved for 24 hours and then fixed in 1% formaldehyde (Thermo Fisher Scientific) for 8 minutes at room temperature. After sonication, chromatin was immunoprecipitated with specific antibodies overnight at 4°C. The antibodies used were anti-H3K4me1 (ab8895, Abcam), anti-GR (catalog 12041, Cell Signaling Technology), and rabbit IgG (catalog 2729, Cell Signaling Technology). Complexes were then immunoprecipitated with protein A magnetic beads for 1 hour at 4°C and reverse-crosslinked at 65°C for 2 hours. The eluted DNA was purified and used as template for qPCR. A 10% input sample was used as control. The enhancer-specific primers, 5′-CTGAGACTGGAGGCTTTTGG-3′ (forward) and 5′-GACATCCCAGTTTCCACTGC-3′ (reverse), were designed using Primer3 software (42 (link)). MYT1 exon 1 primers were purchased from Cell Signaling Technology (catalog 4493).