Western Blotting of Protein Targets

Western blotting was performed according to our previous protocol [26 (link)]. The primary antibodies were anti-METTL14 (#51104S), anti-RB1CC1 (#12436S), anti-LC3B (#3868T) (1:1000, Cell Signaling Technology), and anti-GAPDH (1:2000, 30202ES60, Yeasen, China).

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Liang J., Cai H., Hou C., Song F., Jiang Y., Wang Z., Qiu D., Zhu Y., Wang F., Yu D, & Hou J. (2023). METTL14 inhibits malignant progression of oral squamous cell carcinoma by targeting the autophagy-related gene RB1CC1 in an m6A-IGF2BP2-dependent manner. Clinical Science (London, England : 1979), 137(17), 1373-1389.

Publication 2023

anti-METTL14 anti-LC3B anti-GAPDH

Antibodies Gapdh Mettl14

Corresponding Organization :

Other organizations : Stomatology Hospital, Sun Yat-sen University, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University

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Variable analysis

independent variables

Primary antibodies used: anti-METTL14 (#51104S), anti-RB1CC1 (#12436S), anti-LC3B (#3868T) (1:1000, Cell Signaling Technology), and anti-GAPDH (1:2000, 30202ES60, Yeasen, China)

dependent variables

Protein expression levels of METTL14, RB1CC1, LC3B, and GAPDH

control variables

Western blotting protocol was performed according to a previous protocol [26 (link)]

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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