Western Blot Analysis of Signaling Proteins

Equal amounts of cell lysates were separated on 10% or 12% sodium dodecyl sulfate-polyacrylamide gels. The blotting membranes were probed using antiserum of GAPDH (6C5), MALT1 (EP603Y, Abcam, Cambridge, MA, USA), β-actin (MAB1501), NF-κB p50 (06-886), NF-κB p65 (06-418, Merck Millipore, Burlington, MA, USA), Lamin B1 (D9V6H), IκB-α (#9242), p-IκB-α (#2859, Cell Signaling Technology, Inc. Danvers, MA, USA), NDRG1 (42-6200, Thermo Fisher Scientific Inc.), or BTG2 [31 (link)]. Band intensities were detected by the Western lightning plus-ECL detection system (PerkinElmer Inc, Waltham, MD, USA), recorded using the LuminoGraph II (Atto Corporation, Tokyo, Japan), and analyzed using the GeneTools of ChemiGenius (Syngene, Cambridge, UK).