Cell Viability, Proliferation, and Apoptosis Assays

Cell viability was assayed using either the CellTiter AQueous Non-Radioactive Cell Proliferation Assay (MTS, Huntsville, AL, USA) or CellTiter Luminescent Cell Viability Assay (Promega, Madison, WI, USA) as described previously.^{18 (link)} BrdU incorporation was assayed using a BrdU Cell Proliferation Assay according to the manufacturer's suggested protocol (Calbiochem, Billerica, MA, USA). Real time analysis of cellular proliferation by xCELLligence assay was performed as described previously.^{18 (link)}Apoptosis was determined by nuclear fragmentation assay as described previously.^{54 (link), 55 (link), 56 (link), 57 (link)} Quantifications were performed in triplicate, with each count consisting of at least 300 cells. Caspase 3/7 activation was evaluated by Caspase 3/7 kit (Promega) according to the manufacturer's suggested protocol.

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O'Donnell E.F., Koch D.C., Bisson W.H., Jang H.S, & Kolluri S.K. (2014). The aryl hydrocarbon receptor mediates raloxifene-induced apoptosis in estrogen receptor-negative hepatoma and breast cancer cells. Cell Death & Disease, 5(1), e1038-.

Publication 2014

CellTiter Luminescent Cell Viability Assay Caspase 3/7 kit

Apoptosis Assay Brdu Caspase 3 Caspase 7 Cell viability Cells Cellular proliferation Luminescent assay Promega Radioactive

Corresponding Organization :

Other organizations : Oregon State University

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Variable analysis

independent variables

Cell viability assay method (CellTiter AQueous Non-Radioactive Cell Proliferation Assay (MTS) or CellTiter Luminescent Cell Viability Assay)
BrdU incorporation assay
Real-time cellular proliferation analysis by xCELLligence assay
Apoptosis determination by nuclear fragmentation assay

dependent variables

Cell viability
Cell proliferation (BrdU incorporation and xCELLligence assay)
Apoptosis (nuclear fragmentation)

control variables

Cell culture conditions (not explicitly mentioned)

controls

Positive control: Not specified
Negative control: Not specified

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