The Metasequoia seed cone was collected at the Leuven botanical gardens.
The resistor is a 15-kΩ resistor with a tolerance of 5% and has the four band resistor codes: brown, green, red, and gold, purchased from R&S (RS Components GmbH Hessenring 13b, 64546 Mörfelden-Walldorf).
Figurines are from LEGO™ (Billund, Denmark).
The Chrysolina americana sample was collected approximately at 50° 85′ 82.49″ N, 47° 04′ 25.3″ E.
The Drosophila samples were fixed at − 80 °C to maintain the morphology and fluorescence. The fly strain used in Fig.4 a–d expresses GFP in the eyes in a white-eyed background (genotype: y[1] M{vas-int.Dm}ZH-2A w[*]; Bloomington stock center # 24481). Fly strains used in Fig. 4 e–h were Canton-S (CS), Kyoto stock center # 105666, and w; GlaBC/CyO (Bloomington Drosophila stock center # 6662).
Grids were square mesh EM support grids, 400 copper mesh with 26 μm bars (FCF 400 – Cu – SB Electron Microscopy Science) and a finder grid with 17 μm bars (Agar scientific).
Beads were magnetic Dynabeads 500 with iron core with ~ 5 μm size (Thermo Fisher).
A Pollia dorbignyi [56 ] shell was used for the spectral imaging and was obtained at 42° 21′ 49.7″ N, 3° 09′ 47.2″ E.
Samples were mounted using plasticine.
For Additional file13 : Figure S8, a third instar larva was collected from the food of ongoing fly culture and washed in tap water. The wet larva was stuck to the insect pin by adhesion. For imaging, the larva was exposed to an atmosphere of CO2 to stop it from moving during the acquisition. A 0.2 M NaN3 solution for 30 min was used to relax the muscles [57 ].
Xenopus tropicalis embryos were placed in FEP tubes, with 1.6 mm diameter for imaging. Fixed embryos were imaged in PBS, while living ones were kept in 1/9th diluted Modified frog Ringer (MR: 0.1 M NaCl,1.8 mM KCl, 2.0 mM CaCl2, 1.0 MgCl2, 5.0 mM, HEPES-NaOH (pH 7.6), or 300 mg/l NaHCO3) solution. The tube with the frog embryo was submersed in a buffer containing glass cuvette during acquisition. For the live imaging, a crest3-gfp transgenic reporter line was crossed to the F1 generation, and the offspring was imaged (the transgenic Xenopus line is unpublished data from Schmucker’s lab).
The resistor is a 15-kΩ resistor with a tolerance of 5% and has the four band resistor codes: brown, green, red, and gold, purchased from R&S (RS Components GmbH Hessenring 13b, 64546 Mörfelden-Walldorf).
Figurines are from LEGO™ (Billund, Denmark).
The Chrysolina americana sample was collected approximately at 50° 85′ 82.49″ N, 47° 04′ 25.3″ E.
The Drosophila samples were fixed at − 80 °C to maintain the morphology and fluorescence. The fly strain used in Fig.
Grids were square mesh EM support grids, 400 copper mesh with 26 μm bars (FCF 400 – Cu – SB Electron Microscopy Science) and a finder grid with 17 μm bars (Agar scientific).
Beads were magnetic Dynabeads 500 with iron core with ~ 5 μm size (Thermo Fisher).
A Pollia dorbignyi [56 ] shell was used for the spectral imaging and was obtained at 42° 21′ 49.7″ N, 3° 09′ 47.2″ E.
Samples were mounted using plasticine.
For Additional file
Xenopus tropicalis embryos were placed in FEP tubes, with 1.6 mm diameter for imaging. Fixed embryos were imaged in PBS, while living ones were kept in 1/9th diluted Modified frog Ringer (MR: 0.1 M NaCl,1.8 mM KCl, 2.0 mM CaCl2, 1.0 MgCl2, 5.0 mM, HEPES-NaOH (pH 7.6), or 300 mg/l NaHCO3) solution. The tube with the frog embryo was submersed in a buffer containing glass cuvette during acquisition. For the live imaging, a crest3-gfp transgenic reporter line was crossed to the F1 generation, and the offspring was imaged (the transgenic Xenopus line is unpublished data from Schmucker’s lab).
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