Immunohistochemical Analysis of Xenograft Tumors

Paraffin sections (5 μm) from xenograft tumor samples were deparaffinized in 100% xylene and rehydrated with a decreasing ethanol series and then water. Antigen retrieval was conducted in 0.01 mol/L sodium citrate buffer (pH 6.0) at 100°C for 10 minutes. IHC assay was performed as described previously using supersensitive horseradish peroxidase immunohistochemistry kit (Sangon Biotech, Shanghai, China) [3 (link)] per manufacturer’s instructions with anti-Ki67 antibody (sc-23900, Santa Cruz) and anti-MMP2 antibody (sc-13595, Santa Cruz).

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Zhang H., Wang J., Yu T., Wang J., Lu J, & Yu Z. (2022). Silencing LncRNA CASC9 inhibits proliferation and invasion of colorectal cancer cells by MiR-542-3p/ILK. PLoS ONE, 17(4), e0265901.

Publication 2022

supersensitive horseradish peroxidase immunohistochemistry kit anti-Ki67 antibody (sc-23900,

Anti antibody Antigen Assay Buffer Ethanol Horseradish peroxidase Immunohistochemistry Mmp2 Paraffin Sodium citrate Tumor Xenograft Xylene

Corresponding Organization : Fuzhou Pulmonary Hospital of Fujian

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Variable analysis

independent variables

Antigen retrieval in 0.01 mol/L sodium citrate buffer (pH 6.0) at 100°C for 10 minutes

dependent variables

Expression levels of Ki67 and MMP2 proteins

control variables

Paraffin sections (5 μm) from xenograft tumor samples
Deparaffinization in 100% xylene and rehydration with a decreasing ethanol series and then water

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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