Two topical preparations (10 mg/g and 20 mg/g) were prepared containing the crude ethanolic extract from A. chica leaves mixed with Lanette® cream, as a water-soluble anionic vehicle.
Forty 4-to-6 week old male Swiss Webster mice from the animal facility of the Universidade Estadual do Maranhão (São Luís, Maranhão, Brazil) were used in this experiment. The animals were randomly divided into two groups (n = 20 each). The control group was treated with Lanette® cream and the experimental group was treated with Lanette® cream containing the crude ethanolic extract of A. chica.
For creation of a dorsal skin wound, the animals were anesthetized with an intraperitoneal injection of xylazine hydrochloride (10 to 15 mg/kg) and ketamine hydrochloride (100 to 150 mg/kg). Trichotomy of the dorsal region and demarcation of the skin with a punch was then carried out. Resection of a circular segment of approximately 6 mm diameter was performed, exposing the dorsal fascia. Hemostasis was carried out by digital compression with sterile gauze, with treatment initiated immediately afterwards.
The therapeutic protocol was performed twice a day throughout the experimental period. Transverse and longitudinal diameters of the lesions were measured daily, using a digital caliper, and monitored through photography. Skin wound areas were calculated using the formula A = π x R x r, in which A represents the area, π is equal to 3.14, R represents the largest diameter and r the smallest diameter of the wound.
Forty 4-to-6 week old male Swiss Webster mice from the animal facility of the Universidade Estadual do Maranhão (São Luís, Maranhão, Brazil) were used in this experiment. The animals were randomly divided into two groups (n = 20 each). The control group was treated with Lanette® cream and the experimental group was treated with Lanette® cream containing the crude ethanolic extract of A. chica.
For creation of a dorsal skin wound, the animals were anesthetized with an intraperitoneal injection of xylazine hydrochloride (10 to 15 mg/kg) and ketamine hydrochloride (100 to 150 mg/kg). Trichotomy of the dorsal region and demarcation of the skin with a punch was then carried out. Resection of a circular segment of approximately 6 mm diameter was performed, exposing the dorsal fascia. Hemostasis was carried out by digital compression with sterile gauze, with treatment initiated immediately afterwards.
The therapeutic protocol was performed twice a day throughout the experimental period. Transverse and longitudinal diameters of the lesions were measured daily, using a digital caliper, and monitored through photography. Skin wound areas were calculated using the formula A = π x R x r, in which A represents the area, π is equal to 3.14, R represents the largest diameter and r the smallest diameter of the wound.
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