Genome Sequencing of Monokaryon Ganoderma incarnatum

We isolated protoplast-derived monokaryons from the dikaryotic strain of the G. incarnatum commercial strain CCMJ2665. The monokaryons were obtained as described previously [16 (link)], except that the dikaryotic mycelia were incubated for 240 min at 30 °C in lywallzyme lysing enzyme. The single-nucleated genomic DNA of the G. incarnatum monokaryon strain was then used for genome sequencing and annotation. Genomic DNA was extracted using NuClean Plant Genomic DNA Kits (CWBIO, Beijing, China). The genome of G. incarnatum was sequenced on a PacBio Sequel long-read sequencing platform with a library insert size of 20 kb, at the Engineering Research Center of the Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University (Changchun, China). Raw data were assembled with SMARTdenovo (https://github.com/ruanjue/smartdenovo). The completeness of the genome assembly was evaluated using Core Eukaryotic Genes Mapping Approach (CEGMA) [17 (link)] and Benchmarking Universal Single-Copy Orthologs (BUSCO; [18 (link)]). The whole-genome sequence of G. incarnatum has been deposited in GenBank (in submission). The genome reported in this study has been deposited in GenBank under the accession RZIO00000000.