Immunohistochemical Analysis of Brain Sections

The immunohistochemical analyses were performed as described in ref. ^{61 (link)}. In brief, brain sections were rinsed 5 times in PBS (Sigma-Aldrich P5368-10pak) for 5 min, and then blocked in 10% normal donkey serum and 0.3% Triton X-100 in PBS to minimize nonspecific binding. The sections were then incubated with the primary antibodies (Supplementary Table 4) in the blocking solution for 48 h at 4 °C. The sections were then rinsed in PBS and incubated with a corresponding secondary antibody for 4 h. After the last rinse, the sections were mounted, air dried overnight; and were then sealed with a cover slip in Dako Fluorescence Mounting Medium (S3023, Dako North America, Inc., Carpinteria, CA) and stored at −20 °C.