Immunoblotting (IB) and signal detection was performed as previously described53 (link). The following antibodies were used for IB: anti-KIF21A (orb184767, Biorbyt, diluted 1:1000), anti‐γTubulin (clone GTU‐88, Sigma Aldrich, diluted 1:5000) and respective HRP‐conjugated antibodies (DAKO, diluted 1:5000).
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